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BLAST - Exercise #11

The following sequence contains an open reading frame (ORF) in one of their 6 reading frames.

  1. Use the EMBL-EBI web based sequence tool to translate the sequences below.
    (http://www.ebi.ac.uk/Tools/emboss/transeq/index.html)
    Find the ORF in the correct reading frame (you must select “6” under the frames pull down menu before translating to see all 6 frames).
  2. Once you find the ORF protein sequence, perform a BLAST search at the EcoCyc web site (http://www.ecocyc.org/, located in the search menu) to determine the identity and function of the protein.
  3. Report the correct reading frame (+3, +2, +1, -1, -2, or -3), the translated sequence from the start methionine to the stop codon (you can assume the first methionine in the correct reading frame is the start methionine), the encoded protein name, and a brief description of its function.

 

Sequence #11:

TTTTATCAAAATCAAGAACGGCGTTTGGTTGCGGAGTCCATCCATACTGCCAGCAACAGA
ATCGCACCTTTAACGATATACTGCCAGAAGGTCGGTACATCCATCATACTCATGCCGTTA
TCCAGTGAAGCCATGATAAATGCCCCCATTACTGCTCCGGCAACGCTTCCCACACCGCCA
GCCAGGCTGGTGCCGCCAATCACGCATGCTGCAATTGCGTCCAGTTCGGCGATATTTCCC
GCAGAAGGTGAACCAGCGCCAAGTCGAGAACTAAGGATTAATCCGGCGATGGCTACCATT
AATCCGTTAATCGCGAACACGGCAAGTTTGGTGCGTTCAACGTTAATCCCGGAGAGACGT
GCTGCTTCCAGATTGCCGCCGATGGCATAAATGCGTCGTCCAAATGCCGTCCGCGTTGCC
ATAAACATTCCGCCGAGTAACAGCAACGTCAGCAGCAGAACAGGAGTGGGAACGCCACGG
TAATCATTCAACAGCCAGATTGCGCCTAATACGATGATAGCGGTTAAAGCCTGGCGACCG
ACTACTGCGGTAGAGGCCGGAGACTGTAAACCCAAAGCCTGACGGCGCATTCTTCCGCGC
CATTGCCAACCAACAAAAGCCATTAAGCCAAGCGCGCCAATGATGAAGCCGGTACTGGCG
GGGAGATAGCTTTGCCCAATTTGTGACATCGCGGCGCTGGTGGGGGATACAGTCGTGCCG
TTGGTGATGCCAATGAGTATGCCGCGAAATGCCAACATGCCCGCGAGGGTGACAATAAAT
GAAGGGACTTTACGGTACGCGACCCACCATCCGTTCCAGGCACCGAGAAGCAGTCCCAGA
ACCAGCGTCACAATGATGGTAAGTGGCAAAGGCCAGCCTAACCAGACGTCACAAATCGCC
GCGACGCCACCTAACAGCCCCATCATTGAGCCGACGGAAAGGTCGATTTCAGCAGAAATT
ATGACGAACACCATTCCTACCGCGAGGATGCCGGTAATCGCGGTCTGGCGTAACAGGTTG
GAGACGTTACGGGCGCTTAAGTAGGCACCATCGGTGGTCCAGGTAAAGAACAGCATGATT
GCGATGATAGCTGCAATCATCACGAAGACCTGCAAATTCAGTGATTTCAGCCCGGAGAAG
CCACCGGATGTCGGTACGGCCAATTTCACTTCAGACGGATTGCTTTTCGACATGATGTTC
G