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BLAST - Exercise #10

The following sequence contains an open reading frame (ORF) in one of their 6 reading frames.

  1. Use the EMBL-EBI web based sequence tool to translate the sequences below.
    (http://www.ebi.ac.uk/Tools/emboss/transeq/index.html)
    Find the ORF in the correct reading frame (you must select “6” under the frames pull down menu before translating to see all 6 frames).
  2. Once you find the ORF protein sequence, perform a BLAST search at the EcoCyc web site (http://www.ecocyc.org/, located in the search menu) to determine the identity and function of the protein.
  3. Report the correct reading frame (+3, +2, +1, -1, -2, or -3), the translated sequence from the start methionine to the stop codon (you can assume the first methionine in the correct reading frame is the start methionine), the encoded protein name, and a brief description of its function.

 

Sequence #10:

CGGTTGGGTCAAACGAGTTGTTTACGCTGGTTTGACGGCGGAATGGATAAAGACTCTCGG
TACTTCGCAACAGTGCGGCGTGCCACCATGATACCTTGTTCCGACAGCAAAGAGGTTAAC
TTGCTGTCGCTCAACGGTTTCGCTGGGTTTTCCGCCGCGATTAATTTCTTCACCAGCGCA
CGAATCGCCGTGGAGGAAGCTTCGCCGCCGCCCTCGGTATTGACGTGACTGGAAAAGAAA
TACTTCAGTTCAAAAATGCCTCGTGGACTATGCAGGTATTTTTGCGTGGTCACGCGAGAT
ATCGTCGATTCATGCATTTCGACAGCCTGGGCGATATCGGCCAGTACCATCGGTTTCATA
TATTCTTCACCTTGCTCAAAGAAGGCTTGCTGCTGTTCAACGATACAGCGACTCACGCGC
AGTAGCGTATCGTTACGGCTTTCCAGACTCTTGATCAACCATTTGGCATCCTGCAGATTG
CTGCGGATAAACTGGCTGTCACCATCGTTGCGCGCGTTATTGCACATCGAGGCGTAGTGC
TGGTTGATTTGCAGACGCGGAATGCTGTCACTGTTGAGTTCTACCGTCCAGTGACCGTTA
TGCTTACGCACCAGCACATCTGGAATGACATACTCAGGTTCGCCAGTCTGGATCGACTGC
CCGGGGCGCGGATCGAGCGACTGGATCAGATTGACGGCTTCTTTCAGCACATCTTCTTTC
AGACGCGTGACGCGCATTAAAGTGCGGAAGTCGTGATTGGCTAACAGATCGAGATGATCG
CTAATGATCAGTCTGGCCTCTTCCAGCCACGGCGTGGTCTTATCGAATTGGGAGAGTTGG
ATCAGCAGACAGTCACGCAGATCTTTTGCCGCCACACCGACCGGATCAAACCGTTGGATC
CGCTTAAGGACGGCTTCAACTTCGTCGATGTCGATCTCTTCATCGCCTATACTTTCGAGA
ATATCTTCCAGCGGGACAGTCAGATAACCGGTTTCATCAACGGCATCGACGATAGAGGTA
GCAATAGCGCGGTCAGTGTCGGAAAACGGTGTCAGCTCAACCTGCCACATCAGGTAATCC
TGCAAGGTCTGCGTCGTTTCGCCCTGGTAGACCGGCAGCTCGTCGTCAATGTAGTCACCG
CTGGTGCCGGATGGTGTACCAGCGGTGTAAATGGTGTCCCAACTGGCATCGAGCGGCAGC
TCTTCCGGCATCTCTTTTTGTTCGAGCGCGTCGGCGGTGTCCAGCGTTTCACTGTCTTGC
GTTTCGCGGGTGTCGATTTCTTCATGAGTGTCGATTTGCTCAAGCAGCGGATTACTCTCC
AGCGCCTGCTGTAGCTCCTGCTGAAGTTCCAGCGTCGACAACTGCAACAGACGAATTGCC
TGTTGGAGCTGTGGCGTCATCGCCAGTTGTTGGCTAAGCCTGAGTTGCAAACCTTGCTTC
ATGTTCAGAATC