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Aerobic Respiration - Exercise #1 - Advanced

E. coli has three operons encoding for three distinct cytochrome oxidase enzymes - cytochrome bo oxidasecytochrome bd-1 oxidase and cytochrome bd-2 oxidase

  1. Give the subunit composition for each of the cytochrome oxidases.
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  3. How and which transcription factor(s) regulate expression of cytochrome bo oxidase and cytochrome bd-1 oxidase? What environmental factor(s) regulate gene expression?
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  5. Name two substrates that can serve as electron donors to the cytochrome oxidases via the quinone pool? Name the enzymes that carry out the oxidation reactions.
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  7. You decide to compare and contrast two electron transport chains both initiating from NDH-1.  The electron transport chains differ in their terminal oxidase using either cytochrome bo oxidase or cytochrome bd-1 oxidase. Describe the similarities and differences you find.  Explain why one creates more ATP.