Aerobic Respiration - Exercise #4

Your lab uses the facultative anaerobe E. coli K-12 strain MG1655 as its model organism.  You have been asked to compare and contrast succinate dehydrogenase and glycerol-3-phosphate dehydrogenase, both of which are used for aerobic respiration.

  1. Both dehydrogenase enzymes oxidize their electron donor and donate electrons to which group of intermediate electron carriers? 


  3. In addition, what is the subunit composition of these two enzymes?


  5. Your PI suggests that you knock out all dehydrogenase enzymes in E. coli that are involved in respiration, except for Formate dehydrogenase-N (Fdh-N).  Do you expect that your modified strain would be able to grow in the presence of oxygen? Please explain.