Aerobic Respiration - Exercise #4
Your lab uses the facultative anaerobe E. coli K-12 strain MG1655 as its model organism. You have been asked to compare and contrast succinate dehydrogenase and glycerol-3-phosphate dehydrogenase, both of which are used for aerobic respiration.
- Both dehydrogenase enzymes oxidize their electron donor and donate electrons to which group of intermediate electron carriers?
- In addition, what is the subunit composition of these two enzymes?
- Your PI suggests that you knock out all dehydrogenase enzymes in E. coli that are involved in respiration, except for Formate dehydrogenase-N (Fdh-N). Do you expect that your modified strain would be able to grow in the presence of oxygen? Please explain.