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Aerobic Respiration - Exercise #4

Your lab uses the facultative anaerobe E. coli K-12 strain MG1655 as its model organism.  You have been asked to compare and contrast succinate dehydrogenase and glycerol-3-phosphate dehydrogenase, both of which are used for aerobic respiration.

  1. Both dehydrogenase enzymes oxidize their electron donor and donate electrons to which group of intermediate electron carriers? 
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  3. In addition, what is the subunit composition of these two enzymes?
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  5. Your PI suggests that you knock out all dehydrogenase enzymes in E. coli that are involved in respiration, except for Formate dehydrogenase-N (Fdh-N).  Do you expect that your modified strain would be able to grow in the presence of oxygen? Please explain.