Aerobic Respiration - Exercise #3
Your lab uses the facultative anaerobe E. coli K-12 strain MG1655 as its model organism. You have been asked to study the various dehydrogenases used in aerobic environmental conditions.
- NADH dehydrogenase II is used to oxidize NADH during aerobic respiration. Name two pathways that generate NADH.
- Does this dehydrogenase pump protons to the periplasmic space? If so, how many protons? If not, name the purposes it serves.
- The electrons from the oxidation of NADH (by NADH dehydrogenase) are ultimately used by a reductase complex to reduce O2 to H2O. During aerobic respiration, would E. coli use Nitrate reductase, Cytochrome bo oxidase or DMSO reductase? Explain briefly.