Aerobic Respiration - Exercise #2
Your lab uses the facultative anaerobe E. coli K-12 strain MG1655 as its model organism. You have been asked to determine which terminal cytochrome oxidases are used in varying environmental conditions.
- Which cytochrome terminal oxidase(s) are used during microaerophilic conditions? Which oxidase(s) are used during high oxygen concentrations?
- Please list which genes encode the two different subunit 1s of the terminal oxidases.
- You wish to further study the regulation of the two cytochrome oxidases operons. You notice that Fructose-1-phosphate regulates the cytochrome bo oxidase operons. Explain how it acts and how that affects bo oxidase operon expression.